SALSA MLPA P128 CYP450 probemixbasic research

This SALSA® MLPA® kit is for basic research and intended for experienced MLPA users only! This kit enables you to quantify genes or chromosomal regions in which the occurrence or relevance of copy number changes is not yet well-established. Hence, it will not always provide you with clear cut answers, and interpretation of results can be very complicated.

Application: Cytochrome P450
Region: CYP2D6, CYP2C9, CYP2C19, CYP1B1, CYP3A4, CYP3A5, CYP2E1, CYP1A1, CYP1A2, CYP2A6, CYP2B6, GSTP1, GSTT1 and GSTM1
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Item no. Description Price
P128-025R SALSA MLPA P128 CYP450 probemix – 25 rxn € 243,00€ 194,40
P128-050R SALSA MLPA P128 CYP450 probemix – 50 rxn € 486,00€ 388,80
P128-100R SALSA MLPA P128 CYP450 probemix – 100 rxn € 972,00€ 777,60
EK1-FAM SALSA MLPA EK1 reagent kit – 100 rxn - FAM € 300,00
EK1-Cy5 SALSA MLPA EK1 reagent kit – 100 rxn - Cy5 € 300,00
EK5-FAM SALSA MLPA EK5 reagent kit – 500 rxn - FAM € 1380,00
EK5-Cy5 SALSA MLPA EK5 reagent kit – 500 rxn - Cy5 € 1380,00
To perform MLPA, both a probemix and reagent kit are needed.

Description
General Information: The SALSA MLPA Probemix P128 CYP450 is a research use only (RUO) assay for the detection of deletions or duplications in the CYP2D6, CYP2C9, CYP2C19, CYP1B1, CYP3A4, CYP3A5, CYP2E1, CYP1A1, CYP1A2, CYP2A6, CYP2B6, GSTP1, GSTT1 and GSTM1 genes. Cytochrome P450 enzymes are important in the biosynthesis and degradation of endogenous compounds such as steroids, lipids and vitamins. These enzymes reduce or alter the pharmacologic activity of many drugs and facilitate their elimination. Differences in drug metabolism in the intestine or liver between patients are major contributors to drug response, including adverse effects.
Glutathione transferases are a family of proteins that play an important role in detoxification by catalyzing the conjugation of many compounds with reduced glutathione.

More information is available at https://www.ncbi.nlm.nih.gov/books/NBK1419/

Probemix content: The SALSA MLPA Probemix P128-D1 CYP450 contains 52 MLPA probes with amplification products between 128 and 504 nt. In the normal population several of these genes differ in copy number. For each gene at least two probes are present.

GSTM1 (8 exons), spans ~6 kb of genomic DNA and is located on 1p13.3, 110 Mb from the p-telomere.
CYP1B1 (3 exons), spans ~8.6 kb of genomic DNA and is located on 2p22.2, 38 Mb from p-telomere.
CYP3A4 (13 exons), spans ~27 kb of genomic DNA and is located on 7q22.1, 99 Mb from the p-telomere.
CYP3A5 (13 exons), spans ~32 kb of genomic DNA and is located on 7q22.1, 100 Mb from the p-telomere.
CYP2C19 (9 exons), spans ~93 kb of genomic DNA and is located on 10q23.33, 95 Mb from the p-telomere.
CYP2C9 (9 exons), spans ~51 kb of genomic DNA and is located on 10q23.33, 95 Mb from the p-telomere.
CYP2E1 (9 exons), spans ~12 kb of genomic DNA and is located on 10q26.3, 134 Mb from the p-telomere.
GSTP1 (7 exons), spans ~3 kb of genomic DNA and is located on 11q13.2, 67 Mb from the p-telomere.
CYP1A1 (7 exons), spans ~6 kb of genomic DNA and is located on 15q24.1, 75 Mb from the p-telomere.
CYP1A2 (7 exons), spans ~8 kb of genomic DNA and is located on 15q24.1, 75 Mb from the p-telomere.
CYP2A6 (9 exons), spans ~7 kb of genomic DNA and is located on 19q13.2, 41 Mb from the p-telomere.
CYP2B6 (9 exons), spans ~27 kb of genomic DNA and is located on on 19q13.2, 41 Mb from the p-telomere.
GSTT1 (5 exon), spans ~8 kb of genomic DNA and is located on 22q11.23, 24 Mb from the p-telomere.
CYP2D6 (9 exons), spans ~4.3 kb of genomic DNA and is located on 22q13.2, 42 Mb from the p-telomere.

In addition, twelve reference probes are included in this probemix, detecting different autosomal chromosomal locations. Complete probe sequences and the identity of the genes detected by the reference probes is available online (www.mlpa.com).

This probemix contains nine quality control fragments generating amplification products between 64 and 105 nt: four DNA Quantity Fragments (Q-fragments), two DNA Denaturation Fragments (D-fragments), one benchmark fragment, one chromosome X and one chromosome Y-specific fragment. More information on how to interpret observations on these control fragments can be found in the MLPA General Protocol and online at www.mlpa.com.

Product History
Current version: D1, sold since: 2018-11-28.

Version D1: One probe for CYP2B6 and one for CYP3A5 were removed, in addition one reference probe has been replaced and several probe lengths have been adjusted
Version C1: Nine reference probes have been replaced and one CYP2D6 probe has been removed.
Version B2: QDX2 have been added
Version B1: XY fragments, 2 CYP2C9 probes have been added. CYP2C19 and several reference probes have been replaced.
Version A1: Changes not specified

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