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SALSA MLPA KIT P007 Human chromosomal aberration-3
[http://www.geneclinics.org/profiles/hht/details.html]
Many of the chromosomal aberrations present in human cell lines and cancer cells are numerical changes in copy number of complete chromosomes or specific chromosomal regions. MLPA provides the possibility to detect numerical changes of 40 different sequences in large numbers of samples in an easy to perform reaction requiring only 20 ng of human DNA. SALSA MLPA kit P007 contains probes for 40 different target sequences. Among these are many genes that are often deleted or amplified in various tumors. For example this kit can be used for the detection of chromosomal aberrations in small preneoplastic lesions and the determination of clonality in tumor samples. In addition, the number of chromosomal rearrangements and numerical changes in various cell lines is often very large. The MLPA kits P005, P006 and P007 can be used to prepare a fingerprint of DNA extracted from cell lines, for instance for comparison of supposedly identical cell lines from different laboratories.
Full mix description (pdf)
Last change in probe mix content: Lot 0108 (January 2008) Current Lot Number.: Lot 0108
IMPORTANT NOTICE: MLPA kits are sold by MRC-Holland for research purposes and to demonstrate the possibilities of the MLPA technique. This kit is not CE/FDA certified for use in diagnostic procedures. Salsa MLPA kits are supplied with all necessary buffers and enzymes. Purchase of the Salsa MLPA test kits includes a limited license to use these products for research purposes. The use of this MLPA kit requires a thermocycler with heated lid and sequence type electrophoresis equipment. Different fluorescent PCR primers are available. The MLPA technique has been first described in Nucleic Acid Research 30, e57 (2002)
References 2006-- Frequent gene dosage alterations in stromal cells of epithelial ovarian carcinomas. 2005-- A noninvasive genetic screening test to detect oral preneoplastic lesions. 2005-- Genome profiling of melanocytic tumors using multiplex ligation-dependent probe amplification (MLPA): Its usefulness as an adjunctive diagnostic tool for melanocytic tumors. 2005-- Multiplex ligation-dependent probe amplification for the detection of chromosomal gains and losses in formalin-fixed tissue. 2003-- Delineating genetic pathways of disease progression in head and neck squamous cell carcinoma.
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