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SALSA MLPA KIT P037 CLL 1 & P038 CLL 2
[http://www.geneclinics.org/profiles/hht/details.html]
CHRONIC LYMPHOCYTIC LEUKEMIA (CLL) is the most frequent type of leukemia in the western world and results in the progressive accumulation of mature CD5(+) B lymphocytes in blood and bone marrow of the affected person. Chromosomal translocations are rare events in B-CLL. Copy number changes of certain chromosomal regions are however frequent. Some of these have been found to be highly prognostic markers of this disease. These MLPA probemixes P037 and P038 contain probes for several genomic regions that are recurrently imbalanced in B cell CLL. The P037 CLL MLPA kit 1 and the P038 CLL MLPA kit 2 can be used to determine the loss of TP53 (17p13; 8 probes), the RB1 / DLEU / MIR15-16 region on 13q14 (12 probes), the ATM gene on 11q23 (7 probes, the PTEN gene on 10q23 (2 probes), the MYCN gene (2p24; 3 probes), the 8q24 MYC region (3 probes), the 6q25-26 region (3 probes), the CDKN2A-2B genes on 9p21 (2 probes) as well the presence of trisomy 12 (10 probes) and trisomy 19 (5 probes) in DNA samples obtained from chronic lymphocytic leukemia (CLL). In addition, control probes on various other chromosomes are present. For each region to be analysed, several probes are present. This might be necessary for correct analysis of samples containing lower (30-60%) percentages of tumor cells. Please note that these probemixes have not been used on CLL DNA samples at MRC-Holland. Comments on the performance of this product is greatly appreciated. One SALSA P037-38 CLL MLPA kit contains sufficient reagents for 50 reactions with each of these two probemixes. The P037 and P038 mix are also available separately (Order nr. P037 CLL-1 100 reactions or P038 CLL-2 100 reactions). A third CLL probemix, P042, is in preparation and will contain more 11q probes, as well as 6q11, 3q26 and 14q23 probes. Suggestions for inclusion of specific genes / chromosomal regions are appreciated.
Full mix description (word)
Full mix description (pdf)
Current Lot Number.: P037: lot 0608 P038: lot 0207
IMPORTANT NOTICE: MLPA kits are sold by MRC-Holland for research purposes and to demonstrate the possibilities of the MLPA technique. This kit is not CE/FDA certified for use in diagnostic procedures. Salsa MLPA kits are supplied with all necessary buffers and enzymes. Purchase of the Salsa MLPA test kits includes a limited license to use these products for research purposes. The use of this MLPA kit requires a thermocycler with heated lid and sequence type electrophoresis equipment. Different fluorescent PCR primers are available. The MLPA technique has been first described in Nucleic Acid Research 30, e57 (2002)
References Poster 2007 - F. O’Neill, N.Bown1 E.Wilmore, A.Elliott1 and C.Lowe: Comparison of MLPA and FISH in Chronic Lymphocytic Leukaemia.
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