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SALSA MLPA KIT P084 HNSCC-1
[http://www.geneclinics.org/profiles/hht/details.html]
This SALSA MLPA kit has been developed in collaboration with the Free University of Amsterdam (R.H. Brakenhoff) to detect genetic alterations in the oral cavity and oropharynx. Genes / chromosomal regions targeted by the probes in this P084 probemix include TP53 at 17p13.1; CDKN2A at 9p21; 2q14; 3p22; 4q; 8p22; 8q24; 11q; 13q, 18q, 19p and 20q. In addition several probes have been included for "quiet" regions in HNSCC. This SALSA MLPA kit is designed to detect deletions/duplications of one or more exons of the aforementioned genes. Heterozygote deletions of probe recognition sequences should give a 35-50% reduced relative peak area of the amplification product of that probe. However, mutations and/or polymorphisms very close to the probe ligation site may also result in a reduced relative peak area. Therefore, apparent deletions detected by a single probe always require confirmation by other methods. We have no information on what percentage of defects in these genes is caused by deletions/duplications of complete exons. Please note that most defects in these genes are expected to be small (point) mutations, most of which will not be detected by this MLPA test.
Full mix description (pdf)
Last change in probe mix content: Lot 0804 (August-2004) Current Lot Number.: Lot 0804
IMPORTANT NOTICE: MLPA kits are sold by MRC-Holland for research purposes and to demonstrate the possibilities of the MLPA technique. This kit is not CE/FDA certified for use in diagnostic procedures. Salsa MLPA kits are supplied with all necessary buffers and enzymes. Purchase of the Salsa MLPA test kits includes a limited license to use these products for research purposes. The use of this MLPA kit requires a thermocycler with heated lid and sequence type electrophoresis equipment. Different fluorescent PCR primers are available. The MLPA technique has been first described in Nucleic Acid Research 30, e57 (2002)
References M Worsham, Delineating genetic pathways of disease progression in head and neck squamous cell carcinoma.
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