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SALSA MLPA KIT P088 Oligodendroglioma 1p-19q
[http://www.geneclinics.org/profiles/hht/details.html]
Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas. Gliomas are known to occur in association with several well-defined hereditary tumor syndromes such as neurofibromatosis-1 and NF2, tuberous sclerosis, Li-Fraumeni syndrome, and Turcot syndrome. However, familial clustering of gliomas often occurs in the absence of these tumor syndromes. This SALSA MLPA kit can be used to determine the loss of chromosome arms 1p and 19q in DNA samples obtained from oligodendroglioma tumors. This SALSA MLPA kit is designed to detect deletions/duplications of one or more exons of the 1p and 19q genes. Heterozygote deletions of probe recognition sequences should give a 35-50% reduced relative peak area of the amplification product of that probe. However, mutations and/or polymorphisms very close to the probe ligation site may also result in a reduced relative peak area. Therefore, apparent deletions detected by a single probe always require confirmation by other methods. Please note that most defects in these genes are expected to be small (point) mutations, most of which will not be detected by this MLPA test.
Full mix description (pdf)
Last change in probe mix content: Lot 0608 (June 2008) Current Lot Number.: Lot 0608
IMPORTANT NOTICE: MLPA kits are sold by MRC-Holland for research purposes and to demonstrate the possibilities of the MLPA technique. This kit is not CE/FDA certified for use in diagnostic procedures. Salsa MLPA kits are supplied with all necessary buffers and enzymes. Purchase of the Salsa MLPA test kits includes a limited license to use these products for research purposes. The use of this MLPA kit requires a thermocycler with heated lid and sequence type electrophoresis equipment. Different fluorescent PCR primers are available. The MLPA technique has been first described in Nucleic Acid Research 30, e57 (2002)
References 2006 -- Multiplex ligation-dependent probe amplification: a diagnostic tool for simultaneous identification of different genetic markers in glial tumors. 2005 -- Multiplex ligation-dependent probe amplification for the detection of 1p and 19q chromosomal loss in oligodendroglial tumors.
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