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SALSA MLPA KIT P095 Aneuploidy
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This probe mix included in this kit contains 8 probes each that are specific for chromosome 13, 18, 21 and X sequences, as well as three probes for human chromosome Y target sequences. Salsa MLPA P095 is an improved version of Salsa MLPA P001. Results obtained with the P095 probemix are less sensitive to variation in quality of the DNA samples Aberrant copy numbers of human chromosomes 13, 18, 21, X and Y are not always lethal. Detection of aberrant chromosomal copy number can be performed by standard chromosome analysis or by fluorescent in situ hybridisation. However, these techniques are time consuming and cannot be performed on purified DNA samples. Other techniques used for the detection of trisomies are based on VNTRs and rely on the presence of three different alleles. This MLPA kit is designed to detect a difference in copy of a specific chromosome. Deletions of probe recognition sequences will be apparent by a 35-50% reduced relative peak area of the amplification product of that probe. However, mutations / polymorphisms very close to the probe ligation site may also result in a reduced relative peak area. The exact ligation sites of the MLPA probes are indicated in the description of the MLPA test. We have tried to avoid all polymorphisms apparent from the Genbank databases. Apparent deletions of a single probe will always require confirmation by other methods, this can be due to a pointmutation. .
Full mix description (word)
Full mix description (pdf)
Last Update Probe mix description: Vs. 08; 16-03-2007 Last change in probe mix content: Lot 0505 (May 2005) Current Lot Number.: Lot 0307
IMPORTANT NOTICE: MLPA kits are sold by MRC-Holland for research purposes and to demonstrate the possibilities of the MLPA technique. This kit is not CE/FDA certified for use in diagnostic procedures. Salsa MLPA kits are supplied with all necessary buffers and enzymes. Purchase of the Salsa MLPA test kits includes a limited license to use these products for research purposes. The use of this MLPA kit requires a thermocycler with heated lid and sequence type electrophoresis equipment. Different fluorescent PCR primers are available. The MLPA technique has been first described in Nucleic Acid Research 30, e57 (2002)
References 2006 -- Double trisomy in spontaneous miscarriages: cytogenetic and molecular approach. 2006-- MLPA as a screening of aneuploidy and unbalanced chromosomal rearrangements in spontaneous miscarriages 2005-- Automatic analysis of multiplex ligation-dependent probe amplification products (exemplified by a commercial kit for prenatal aneuploidy detection). 2005-- Rapid detection of chromosomal aneuploidies in uncultured amniocytes by multiplex ligation-dependent probe amplification (MLPA). 2005-- Computer-assisted prenatal aneuploidy screening for chromosome 13, 18, 21, X and Y based on multiplex ligation-dependent probe amplification (MLPA) 2003-- Rapid, high throughput prenatal detection of aneuploidy using a novel quantitative method (MLPA)
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