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SALSA MLPA KIT P118 WT1
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=OMIM&cmd=Limits]
Defects of the WT1 gene on chromosome 11p13 are the cause of Wilms' tumors (MIM194070), which are characterized by nephroblastoma of childhood. The WT1 gene product is required for normal formation of the genitourinary system and mesothelial tissues. Mutations in WT1 have also been observed in patients with Denys-Drash syndrome (MIM194080), Frasier syndrome (MIM136680) and isolated diffuse mesangial sclerosis (MIM256370). Mutations in the WT1 gene are observed in only 5% of the sporadic Wilms tumors. However more than 90% of patients with Denys-Drash syndrome, which includes Wilms tumor, carry constitutional intragenic WT1 mutations. In addition, large chromosomal deletions including the WT1 gene cause WAGR syndrome (MIM194072). The WT1 gene has 10 exons, spanning approximately 48 Kb of genomic sequence. The gene product is often overexpressed in leukemias and various types of solid tumors. This P118 WT1 kit contains MLPA probes for all 10 WT1 exons. Two probes are present for exon 10 sequences and two probes have been included for the WT1 exon 1 + promotor region. In addition, several probes are present for other genes in the 11p13 region which are often codeleted with WT1 in WAGR syndrome. More probes for the PAX6 gene and the 11p13 WAGR region are present in the P229 PAX6 probemix.
Full mix description (word)
Full mix description (pdf)
Last change in probe mix content: Lot 1106 (November 2006) Current Lot Number.: Lot 1106
IMPORTANT NOTICE: MLPA kits are sold by MRC-Holland for research purposes and to demonstrate the possibilities of the MLPA technique. This kit is not CE/FDA certified for use in diagnostic procedures. Salsa MLPA kits are supplied with all necessary buffers and enzymes. Purchase of the Salsa MLPA test kits includes a limited license to use these products for research purposes. The use of this MLPA kit requires a thermocycler with heated lid and sequence type electrophoresis equipment. Different fluorescent PCR primers are available. The MLPA technique has been first described in Nucleic Acid Research 30, e57 (2002)
References
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