[./productpagepag.html]
[./indexpag.html]
[./support_pagepag.html]
[./contactpagepag.html]
Copyright © 2005 - MRC-Holland
[./indexpag.html]
Home
-
[./productpagepag.html]
Products
-
[./support_pagepag.html]
Support
-
[./article_pagepag.html]
Articles
-
[./contactpagepag.html]
contact
SALSA MLPA KIT P190 CHEK2
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=OMIM&cmd=Limits]
This MLPA kit is designed to detect deletions of one or more exons of the CHEK2 gene. CHEK2, encoding a cell-cycle checkpoint kinase, is a strong candidate gene for cancer susceptibility. Double-strand DNA breaks lead to activation of CHEK2 through ATM phosphorylation. CHEK2 plays an important role in cell cycle regulation and DNA damage repair, processes that are central in prevention of tumor development. The CHEK2 1100delC mutation leads to premature termination and abolishes kinase activity (Einarsdottir et al., 2006; Isinger et al., 2006). This P190 CHEK2 kit contains 15 MLPA probes for CHEK2. In addition, one probe is located in the promoter region of the gene and one probe is specific for the CHEK2 1100delC mutation. This probe will only give a signal when the mutation is present! Furthermore, 9 ATM, 6 PTEN probes and two probes in the 22q12 region just upstream of CHEK2 gene are included. These latter probes are specific for the HSC20 gene and the XBP1 gene. In addition, one BRCA1 and one BRCA2 probe is included which are different from the probes in the P002 BRCA1, P087 BRCA1 and P045 BRCA2 probemixes, as well as two TP53 probes and several control probes on loci that have not been implemented in hereditary breast cancer.
Full mix description (word)
Full mix description (pdf)
IMPORTANT NOTICE: MLPA kits are sold by MRC-Holland for research purposes and to demonstrate the possibilities of the MLPA technique. This kit is not CE/FDA certified for use in diagnostic procedures. Salsa MLPA kits are supplied with all necessary buffers and enzymes. Purchase of the Salsa MLPA test kits includes a limited license to use these products for research purposes. The use of this MLPA kit requires a thermocycler with heated lid and sequence type electrophoresis equipment. Different fluorescent PCR primers are available. The MLPA technique has been first described in Nucleic Acid Research 30, e57 (2002)
References
[./order_infopag.html]
[./products_prenatal_and_postnatalpag.html]
[./products_hereditary_cancer_researchpag.html]
[./products_various_syndromespag.html]
[./products_tumor_characterisationpag.html]
[./products_mrna_analysispag.html]
[./products_methylation_specificpag.html]
[./products_otherpag.html]
[./products_pharmacogeneticspag.html]
[./mlpapricelistpag.html]
Last change in probemix content Current lot number
: lot 0806 (August 2006) : lot 0107
[Web Creator] [LMSOFT]