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SALSA MLPA KIT P264 Human telomere-9
[http://www.geneclinics.org/profiles/hht/details.html]
This P264-A1 Human telomere-9 probemix can be used to confirm and further characterise abnormalities detected by SALSA MLPA kit P036 Human telomere-3 and/or SALSA MLPA kits P069 / P070 Human telomere. This SALSA MLPA P264 probemix contains: 14 probes in the terminal 5.8 Mb of chromosome arm 1q. 12 probes in the terminal 5.0 Mb of chromosome arm 2q. 11 probes in the terminal 4.7 Mb of chromosome arm 3q. 8 probes in the terminal 5.1 Mb of chromosome arm 4q. SALSA MLPA kits P036 and P069/P070 each contain one MLPA probe for each subtelomeric region. Most of these probes detect a well-characterised gene close to the telomere. The probes in P036 and P069/P070 are different, allowing results obtained with one probemix to be confirmed by the other. The majority of probes in P069 are identical to the P070 probes. However, in contrast to P070, no probes for the acrocentric chromosome arms 13p, 14p, 15p, 21p and 22p are present in P069. We recommend to use SALSA MLPA kit P036 Human telomere-3 for primary screening of the subtelomeres. Results obtained with P036 can be confirmed and further characterised with a follow up kit for specific telomere analysis, such as this P264 Telomere-9 kit.
Full mix description (pdf)
IMPORTANT NOTICE: MLPA kits are sold by MRC-Holland for research purposes and to demonstrate the possibilities of the MLPA technique. This kit is not CE/FDA certified for use in diagnostic procedures. Salsa MLPA kits are supplied with all necessary buffers and enzymes. Purchase of the Salsa MLPA test kits includes a limited license to use these products for research purposes. The use of this MLPA kit requires a thermocycler with heated lid and sequence type electrophoresis equipment. Different fluorescent PCR primers are available. The MLPA technique has been first described in Nucleic Acid Research 30, e57 (2002)
References
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Last change in probemix content Current lot number
: lot 0408 (April 2008) : lot 0408
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