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SALSA MLPA KIT RM002 Mouse apoptosis
[http://www.geneclinics.org/profiles/hht/details.html]
This SALSA MLPA RM002 Apoptosis mRNA probemix for mice mRNA contains several probes specific for Mus musculus mRNAs that are important for regulating the apoptosis pathways. The total probemix contains 42 different probes, specific for 40 different genes, among which probes for BAX, BAK1, BCL2, BID and several BIRC mRNAs. MLPA analysis of RNA requires a minimum of only 10 ng total RNA. Strong signals of probes specific for abundant mRNAs can be reduced by inclusion of a competitor oligonucleotide containing the same hybridizing sequence as the synthetic probe oligonucleotides, but lacking the PCR primer sequence. In this RM002 mix, the probe signals of the B2M mRNAs has been deliberately reduced by the inclusion of a competitor oligonucleotide. An MLPA reaction with the use of this probe mix on samples of mouse total RNA will result in a mixture of up to 42 amplification products that can be separated by capillary electrophoresis. The length of each amplification product indicates the probe from which it was derived. The relative amount of each amplification product reflects the relative amount of each probe target sequence in the sample analysed.
Full mix description (word)
Full mix description (pdf)
Last change in probe mix content: Lot 0507 (May 2007) Current Lot Number.: Lot 0507
IMPORTANT NOTICE: MLPA kits are sold by MRC-Holland for research purposes and to demonstrate the possibilities of the MLPA technique. This kit is not CE/FDA certified for use in diagnostic procedures. Salsa MLPA kits are supplied with all necessary buffers and enzymes. Purchase of the Salsa MLPA test kits includes a limited license to use these products for research purposes. The use of this MLPA kit requires a thermocycler with heated lid and sequence type electrophoresis equipment. Different fluorescent PCR primers are available. The MLPA technique has been first described in Nucleic Acid Research 30, e57 (2002)
References
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